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The research objective was to determine the effectiveness of NPK and bio urine fertilizers on soil chemical properties, nutrient uptake, and rice yield. The design used in this study was a randomized block design (RAK = Randomize Complete Block Design) with ten types of treatment and repeated three times. The treatment is a combination of several levels of NPK fertilizer and bio urine fertilizer. The results showed that NPK fertilizer application with bio urine significantly affected pH, total N, available P and K, N, P, K absorption, strawweight, dry grain weight, and 1000 grain weight. Application of NPK fertilizer combined with bio urine fertilizer can increase several elements such as organic C, total N, available P, K, N, P, and K uptake in rice plants. The highest yield of rice obtained in the treatment of 75% NPK fertilizer + 30 ml bio urine, namely 5.65 t ha-1, and the lowest product obtained in the farmer (control) method of treatment, 4.01 t ha-1. The Relative Agronomic Effectiveness (RAE) value is 165.66%. The use of fertilizer at this dosage is more effective than standard fertilizers, and the economic test value of fertilizers is around 1.42; it is suitable for use in rice farming.