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The aim is to study a consortium of microorganisms during the bovine cattle finger and hoof pathology by transmission electron microscopy. Material for the study was taken from phalanges of bovine cattle from sick untreated animals at the boundary of healthy and necrotic tissues. Gram stained smears were examined under a light microscope; micro-organisms were identified by bacteriological examination and bioassay on laboratory animals. For ultrastructural studies, samples were prepared according to modified electron microscopic techniques. Transmission electron microscopy detected areas of metabolically heterogeneous biofilm consisting of different microorganisms in necrotic tissue samples (Fusobacterium necrophorum, Streptococcus spp., Staphilococcus spp., Clostridium spp., Treponeme spp., Proteus vulgaris, Escherichia coli, Pseudomonas spp. and so on), among which Fusobacterium necrophorum prevails. The composition of the bacterial biotope determines the specificity and nosology of the disease. Bacterial cells within biofilms show signs of a high degree of adhesion and the presence of pathogenicity factors: flagella, pili, extracellular polymeric matrix, outer membrane vesicles, intercellular signalling. Taken together, these traits can correlate with their degree of virulence.