Study of Desensitizing Effect of the Product Based on Phytogenic and Microbial Substances

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Dina T. Sharifullina, Ruslan R. Gainullin, Mulanur M. Shakurov, Rimma V. Nefedova, Edie M. Plotnikova, Ekaterina N. Mayorova, Ramzi N. Nizamov, Kamil T. Ishmukhametov, Timur R. Gaynutdinov, Konstantin N. Vagin, Gulnaz I. Rakhmatullina, Zilya R. Kamalova,

Résumé

Considering that the development of autosensitization plays an important role in pathogenesis of the radiation sickness and that the desensitizing drug therapy appears to be efficient for fighting against autoallergic processes in the radiation sickness, these researches were conducted with the aim to study desensitizing properties of the complex product based on phytogenic and microbial substances. The reference extract of potato exposed at the dose of 400 Gy and containing the natural radiomimetic, the quinoid radiotoxin, was used as an offending allergen. The allergic properties of the obtained product was assessed in the experiment conducted on the laboratory animals using the method of labial reaction (LR) according to Freund J. and Stone S. by injecting the allergen into the upper lip skin. The experiments were conducted on 40 guinea pigs and 100 white mice which in 24 hours after irradiation at LD 50 and LD100 were subject to the desensitizing therapy by single subcutaneous administration of the complex product - 0.5% suspension of turmeric powder with bifid bacterium (B.bifidum) culture liquid. The efficacy of the therapy with a potential desensitizing agent for the test subjects was assessed by the degree of inhibition of proinflammatory cytokines - interleukine 4 (IL-4) that is responsible for the development of the allergenic response, suppression of IG E-antibodies, correction of passive cutaneous anaphylaxis (PCA) indicators and inhibition of labial reaction, normalization of immunoregulatory index CD4+/CD8+. The use of the complex product on the basis of phytogenic (turmeric) and microbial (B.bifidum culture liquid) substances after irradiation had a considerable impact on the development of the postradiational autoallergy by reducing the allergic index, reliably reducing the neutrophil damage index (NDI test), inhibiting the synthesis of proinflammatory cytokine (IL-4), increasing the synthesis of specific (“nonprecipitable”) antibodies (IgG), decreasing the level of skin-sensitizing Ig E-antibodies and restoring the faulty subpopulation immunoregulatory index Th/Ts (CD4/CD8). Therefore, a new radiation protective medicinal product was developed on the basis of phytogenic and microbial substances, it has the hyposensitization action achieved by inhibition of radiation-induced proinflammatory cytokines (IL-4), suppression of IgE-response in case of drastic strengthening of synthesis of allergen-specific (“nonprecipitable”) antibodies and by inhibiting the passive cutaneous anaphylaxis (PCA) indicators in the radiosensitized animals. 

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