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This research discusses the study of water quality in the springs at Tirtha Empul Temple, in this study the water source in question is a clear water source that emerges from the ground called kalebutan water. Kalebutan water is usually used as a source of drinking water, melukat and for spiritual activities carried out by the people of Manukaya Village, Tampaksiring, Gianyar Regency in particular and the people of Bali and outside Bali in general. aims to determine the quality of panglukatan water in Pura Tirtha Empul's spring which is taken at different times, namely morning, noon and night, in terms of physical parameters (temperature, smell, taste, and color) and water chemistry parameters. (pH, presence of Cl- ions, and presence of metals such as Fe, Mn, Pb, Na, Mg). The sampling technique used was purposive sampling. The instrument used to determine the quality of Panglukatan water at the spring at Tirtha Empul Temple is the standard of drinking water quality. The results showed that the Panglukatan water samples from the spring at Tirtha Empul Temple taken at different times (morning, noon, and night) were all of good quality in terms of physical parameters (temperature, smell, taste, and color) and chemical parameters. water (pH, presence of Cl- ions, and presence of metals such as Fe, Mn, Pb, Na, Mg). Physically, the water source at Tirtha Empul Temple is suitable for use and the good time that the community can do (pamedek) is in the morning from 06.00-10.00.