Agricultural Products Decontamination from Natural Flora by Gamma-Irradiation

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Yagafar M. Kurbangaleev, Konstantin N. Vagin, Timur R. Gaynutdinov, Nikolaу M. Vasilevsky, Eduard I. Semenov, Gulnaz I. Rakhmatullina, Fanil R. Vafin, Farit H. Kalimullin, Liliya Y. Gabdrakhmanova, Ekaterina N. Mayorova, Sergey Yu. Smolentsev, Kamil T. Is

Résumé

The work studies the degree of radiation decontamination of crop and livestock products from natural flora using the following cultures: nonsporeforming E. сoli, sporeforming B. subtilis, and mold fungus Aspergillus flavus, determines the general toxicity and biological full-value of the irradiated products (meat and grain). In the radiation resistance experiments, the weighs of microbial bodies and fungus spores were irradiated in 6 different doses from 5 to 30 kGy at the interval of 5 kGy. The general toxicity study of meat and grain was performed on 1-10 days after irradiation in the doses of 20 kGy and 25 kGy, respectively, on the test subjects: Stylonychiamytilus, white mice, and rabbits. To assess the biological full-value of these products, on 1-10 days after irradiation, they were given to the weanling rats according to the statural-weight method.  The bacteriological studies showed that the full sterilization of the weigh of E. сoli  КВ-2 was observed in the gamma-ray dose of 15 kGy, B. subtilis-3 – in the dose of 25 kGy, and mold fungus Aspergillus flavus-2610 in the dose of 25 kGy. That’s why to assess irradiated feed (meat and grain) safety, these doses were used.  The irradiation of the contaminated barley showed that the fungus radiation resistance on the contaminated product is to some extent lower than on the pure culture. Full sterilization of the contaminated grain under the specified conditions with a dose of 20 kGy. The general toxicity study results of irradiated meat (lamb) and grain (oats) evidenced the non-toxicity of the products irradiated in the doses specified. Daily feeding of meat and grain irradiated in the doses of 20 kGy and 25 kGy, respectively within 28 days, did not cause considerable changes in clinical, haematological, and pathoanatomical indicators of the organism of the young rats, as well as their growth intensity as compared to the control subjects.   The radiation resistance experiments of the weighs of microbial bodies and fungus spores showed full sterilization of the weigh of culture E. сoli pcs. КВ-2 was observed in the gamma-ray dose of 15 kGy, B. subtilis-3 – in the dose of 25 kGy, and mold fungus Aspergillus flavus-2610 in the dose of 25 kGy. The general toxicity and biological full-value studies of meat and grain on 1-10 days after irradiation in the doses of 20 kGy and 25 kGy, respectively, evidence the irradiated products' biological full-value and safety. 

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