Determination of Pooled Serum as a Better and Cheap Method for Internal Quality Control in Clinical Laboratory

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Shahzad Ali Jiskani, Ali Akbar Pirzado, Muhammad Wasif Saleem, Lubna Naz, Anam Shaikh, Noreen Atzaz


Background: Material for quality control (QC) is consumed rapidly in clinical laboratories after implementation of automation, in order to maintain the quality and monitor the performance. For many developing countries, usage of commercial material for quality control is not affordable due to high cost and non – availability. Preparation and consumption of in-house quality control (QC) pooled serum can be utilized as a purposeful and cost-effective method.

Objective: The main objective of this study is to determine the efficacy of in-house built pooled internal quality control as compared to commercial internal quality control.

Methods and Materials: Left-over serum samples of healthy subjects were pooled using a glass jar by using ethanediol as a preservative, followed by deep freezing at -20OC. 30 aliquots, each having 100 microliters, were prepared from pooled serum. One aliquot of pooled serum along with commercial internal quality control was analyzed daily and consecutively for 30 days for commonly used clinical parameters including urea, creatining, glucose, triglycerides, cholesterol, high-density lipoprotein, total protein, calcium, albumin amylase and liver enzymes. Mean, CV% and standard deviation were calculated for pooled serum samples and commercial samples.

Results: Mean, CV% and standard deviation were statistically significant for cholesterol, triglycerides, glucose, AST, ALT, calcium, alkaline phosphatise and amylase between two groups.

Conclusion: Pooled serum internal quality control is better than commercial internal quality control. Due to less matrix effect, there was stability in pooled serum. Additionally, less variation was observed in pooled serum internal quality control.

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